If the workstation is used for internet access or purposes other than those specified by bd biosciences, it is the buyerusers responsibility to install and maintain uptodate virus protection software. Starting up the cytometer follow these steps to start up your bd facsaria cytometer. Bd facsaria iii users guide core facility flow cytometry. The bd facsaria iii sorter is built on the solid foundation of patented technologies, superior multicolor performance, and legendary easeofuse that opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. Refer to the sys refer to the tlink console user manual for details. Bd facsaria quick reference guide use this quick reference guide once you are familiar with the procedures in the bd facsaria user s guide.
With its fixedoptics design and digital electronics, the bd facsaria iii flow cytometer enables multicolor analysis of up to 18 fluorescent and two scatter options at a time. The bd facsaria iii is built on the solid foundation of patented technologies, superior multicolor performance, and legendary easeofuse that has led to the unparalleled success of the bd facsaria. It is the responsibility of the buyeruser to ensure that all added electronic files including software and transport media are virus free. Bd facsaria fusion manual 227 amici del golden retriever. Users manual hardware edition jy997d28701 this manual contains hardware explanations of wiring, installation and specifications for the fx3uc series programmable controllers. Bd facsaria user guide bd facsaria ii has three lasers. Bd facsariaii has fixedalignment cuvette flow cell, which allows achieving superior fluorescence sensitivity. If you decide to go with the aria iii, just make sure that you get the bigger tip and a chiller. That means the fluorochromes pecy7 and apccy7 though emit in the same range in 78060, but since they excited essentially by different lasers, the emmision spectra will not interfer each other.
Analysis and sorting of cells with facsaria ii flow cytometer 10. Check that the air line and the blue fluidics line is connected to the sheath tank. Learn about bd life sciences diagnostic systems qcpi manuals. Bd biosciences facsdiva software features bd facsdiva. A bd facsaria iii cytometer report includes the following additional information. Table 1 lists symbols that are used in this manual or on safety labels to alert you to a potential hazard. Patented bd facs accudrop technology assists the user to see the best drop. Bd facsaria iii cell sorter user s guides and manuals. Please login to for documentation and training materials.
Bd facsaria quick reference guide biosciences area. A mask is a region of the stream which is monitored for the presence of cells of interest defined by bd biosciences facsaria training manual. We have 1 bd facsaria manual available for free pdf download. Keep this safety information available for future reference and for new users. Check that the sheath tank is full and the waste tank is empty.
Starting up the cytometer follow these steps to start up your bd facsaria ii cytometer. Laser safety lasers or laser systems emit intense, coherent electromagnetic radiation that. Pathology flow cytometry facility sop university of washington page 2 table of contents i. Bd facsaria quick reference guide use this quick reference guide once you are familiar with the procedures in the bd facsaria users guide. The new bd facsaria ii cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. For more details, refer to chapter 4 in the users guide. Bd facsaria ii users guide the bd facsaria ii cytometer uses lowpowered, aircooled and solidstate lasers that do not have special power or cooling requirements.
Bd facsaria iii system is even more powerful, dependable, and easy to use. Ensure that both vent caps are tightened before installing. Operate the instrument only as directed in the users guide. Fx programming manual ii jy992d88101 this manual contains instruction explanations for the fx1s, fx1n, fx2n and fx2nc series programmable controllers. New worksheet features create headers and footers on the worksheet, including cytometer name, experiment name, user name, date and time, bd facsdiva software. Bd facsaria fusion fluorescence activated cell sorter. A biosafety cabinetenclosed highspeed cell sorting instrument equipped with 375nm, 405nm, 445nm, 488nm, 561nm, and 640nm lasers.
Bd serves and supports our customers with full offerings beyond our individual products, enabling integrated solutions across healthcare systems to comprehensively improve care and lower costs. The following figure shows the steps for daily workflow using the bd facsaria users guide assumes you have a working knowledge of basic. The heart of this cell sorting revolution is the true fixedalignment flow cell, gelcoupled to the collection optics. Add 250 ml of bleach to the empty waste tank if necessary. You will want to check the plates to make sure they are dry and clean before you turn on the stream. The fluidics and optical systems are precisely integrated to maximize signal. Micropipettes are made of ultrathin glass capillaries coupled to an aspiration and dispensation unit. Implemented 09192007 102007 ciiiv aar manual of standards and recommended practices specifications for tank cars section ciii table of contents see back of book for aar forms for reproduction by the proponents. Facsaria ii users guide and page number for additional information. The bd facsaria users guide assumes you have a working knowledge of basic.
Bd facsaria users guide national center for xray tomography. Bd facsaria ii quick reference manual pdf download. Turn on the stream and allow time for it to stabilize. Adjust the frequency freq between 27 and 30 to identify the highest breakoff, where the endportion of the breaking stream can still be observed. Several default entries are provided that may be utilized until they are deleted andor until new initials and identification codes are entered into the system. Bd facsaria cellsorting system from bd biosciences.
Masks divide cells into 32 equal segments, and choosing different types of available masks determines how drops will be deflected if a sorting conflict occurs bd biosciences facsaria training manual. If you are a registered library contact, please login with your support. Facsaria protocol littman lab version 040208 elke kurz nicolas manel. Cells can be sorted into tubes or multiwell plates. Sims iii log in screen test users must enter their user name and password. Fusion nozzle are label ms aria ii nozzle are label m do not switch nozzle between instruments. Refer to figure 12 in the system iii installation manual. This guide shows a workflow that uses application settings. Samples preparation filter sample before sorting 70 or 100 nm filter dont dilute sample too much, as more dense as faster the sort. Facsaria sorp standard operation protocol basic operation. Ensure the flow cell access door is raised and the sort chamber door is open.
Bd the bd facsaria iii users guide assumes you have a working knowledge of. See determining the drop delay manual method on page 172 for more information. The bd facsaria is new generation benchtop highspeed flow cytometry cell sorter. Stream breakoff and droplet formation 100m nozzle a. Setup on page 1 in the bd facsdiva software reference manual. The software interface that comes with it is not exactly userfriendly and the manual is too expansive and too general. Adjust the frequency freq between 27 and 30 to identify the highest breakoff, where the end. Export in a subfolder named after the username you use to. Facsaria fusion sorp quick reference guide childrens. Crossinstrument and crosssite standardization using bd facsdiva softwares custom application settings. Text and keyboard conventions are shown in table 2.
Cytometry facsaria not responding purdue university. Bd facsariaii delivers highspeed multicolor sorting with digital acquisition rates of up to 20,000 eventssecond. Julie julie nelson flow cytometry core facility center for tropical and emerging global disease university of georgia jnelson at uga. Manual of standard operating procedures user registration and safe working practices bd facsaria iii cell sorting in h581c.
For applicatio nspecific instructions, refer to the individual application guides and information supplied with the reagents. For a full description of the cytometer status report, see the bd facsdiva software reference manual. It accommodates up to six aircooled lasers and eighteen fluorescent detectors. I will add some more information to the johns answer. Fsc, ssc, 2 blue or 445nm, 5 yellowgreen, 6 violet, and 3 red. However, you can create new modes and then select them from the precision mode menu. The bd facsaria iii users guide assumes you have a bd facsdiva software quick reference guide for bd facsaria cell sorters. Micromanipulators for manual cell picking typically consist of an inverted microscope combined with micropipettes movable through motorized mechanical stages. The properties measured include a particles relative size, relative granularity or internal complexity, and relative. Bd lsrfortessa and bd facsaria iii openbis documentation. Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they. Each laser has its own filters for detectiong emmision spectrum. A copy is at the end of this manual, for the new user to be certified to enter the core or work independently in the facility.